Position of the reduced mycorrhizal colonisation (<Emphasis Type="Italic">Rmc</Emphasis>) locus on the tomato genome map |
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Authors: | Nicholas J Larkan Sally E Smith Susan J Barker |
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Institution: | (1) School of Plant Biology M084, Centre for Soil–Plant Interactions, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia;(2) Soil and Land Systems, School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA, 5005, Australia;(3) Centre for Soil–Plant Interactions, Perth, Australia;(4) Present address: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N0X2, Canada |
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Abstract: | Our research aims to investigate the molecular communication between land plants and arbuscular mycorrhizal (AM) fungi in
the establishment of symbiosis. We have identified a mutation in the facultative AM host tomato, which we named rmc. Plants that are homozygous for rmc no longer host most AM fungi. The mutation also affects the interaction of tomato with root knot nematode and Fusarium wilt.
However, the function/s encoded by the intact Rmc locus is/are unknown. To clone and sequence the gene or genes that comprise the Rmc locus, we have initiated a positional cloning project. In this paper, we report the construction of mapping populations and
use of molecular markers from the published genome map to identify the location of Rmc on tomato chromosome 8. Nucleotide binding site-leucine rich repeat resistance genes, reported to reside in the same region
of that chromosome, provided insufficient differences to develop cleaved amplified polymorphic sequence markers. Therefore,
we were unable to map these sequences in relation to rmc. Our results potentiate future work to identify the Rmc function and to determine the genetic basis for the multiple plant-microbe interaction functions that the rmc mutation has defined. |
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Keywords: | Arbuscular mycorrhiza Mutant Bulked segregant analysis Solanum lycopersicum Microsatellite RFLP NBS-LRR R-genes |
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