Measurement of PGH synthase activity using a pan-specific monoclonal antibody |
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| Authors: | E R Hall D S Linthicum G Townsend B J Patterson K F Frasier-Scott |
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| Affiliation: | Department of Internal Medicine and Pharmacology, University of Texas Medical School, Houston. |
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| Abstract: | A pan-specific monoclonal antibody that recognizes a variety of prostaglandin moieties and does not recognize arachidonic acid or the hydroxy-eicosatetraenoic acids was used to assess the general ability of a tissue to produce prostaglandins. Although this assay does not give a quantitative measure of PGH synthase activity, it does provide a sensitive and convenient means of screening a large number of samples for prostaglandin production. |
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