我国南方不同产量的八角树内生真菌群落研究

八角Illicium verum原产于我国华南和越南北部,是我国传统的香料和药用植物。内生真菌可提高植物生长与产量。然而,八角的产果量与其内生真菌间的关系尚未有人报道。为揭示内生真菌与八角产量的关系,本文分析了广西壮族自治区不同八角产量植株的内生真菌定殖率和群落组成。从60棵植株的叶片中分离到412株内生真菌,并根据形态学和分子生物学方法将其鉴定为22个真菌分类单元。内生真菌群落的优势类群分别为Colletotrichum gloeosporioides、Dermateaceae sp.和Phyllosticta sp. 1。内生真菌定殖率的变化范围为6%-100%,平均值为41%。内生真菌的定殖率与八角产量的回归分析表明,在较低和中等产量的八角植株中,内生真菌定殖率与产量呈正相关,而在高产的八角植株中,内生真菌定殖率与产量呈负相关。

Endophytic fungal community of Illicium verum with different star anise yields in southern China

Chinese anise (Illicium verum), a traditional spice and medicinal plant in China, is endemic in southern China and northern Vietnam. Endophytic fungi may play an important role in promoting plant growth and yield, however, their relationship with star anise yield of I. verum remains enigmatic. In this study, foliar endophytic fungi from I. verum trees with different star anise yields were investigated in order to reveal the relationship between endophytic fungal incidence and star anise yield in Guangxi, southern China. A total of 412 fungal strains were isolated from 60 individuals of I. verum, and 22 fungal taxa were identified based on morphology and molecular biological techniques. Colletotrichum gloeosporioides, Dermateaceae sp., and Phyllosticta sp. 1 were found to be dominant in the endophytic fungal community. The mean colonization rate of endophytic fungi was 41%, with a range from 6 to 100%. Regression analysis showed that endophytic fungal colonization rate was positively correlated with star anise yield in I. verum individuals with low and moderate yield, but negatively correlated with star anise yield in individuals with high yield.