Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland |
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Authors: | Jakubowska Agata van Oers Monique M Ziemnicka Jadwiga Lipa Jerzy J Vlak Just M |
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Institution: | Laboratory of Virology, Wageningen University, Binnenhaven 11, Wageningen, 6709 PD, The Netherlands. |
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Abstract: | The turnip moth, Agrotis segetum (Lepidoptera, Noctuidae), is an important pest insect in Europe, Asia, and Africa. We have genetically characterized and classified a nucleopolyhedrovirus isolated from A. segetum larvae in Poland (AgseNPV-P). The restriction pattern of AgseNPV-P was distinct from an isolate from England/France (AgseNPV-UK and AgseNPV-F). Sequence analysis of three conserved baculovirus genes, polyhedrin, lef-8 and pif-2, revealed that AgseNPV-P differs substantially from the already described NPVs isolated from A. segetum and possibly represents a new NPV species. Phylogenetic analysis placed AgseNPV-P among group II NPVs and showed the closest relationship to Agrotis ipsilon (Agip) NPV and Spodoptera exigua (Se) MNPV. |
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Keywords: | Agrotis segetum Nucleopolyhedrovirus AsMNPV Phylogeny Taxonomy |
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