Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from <Emphasis Type="Italic">Geobacillus stearothermophilus</Emphasis> strain 18 |
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Authors: | Milda Stuknyte Simone Guglielmetti Diego Mora Nomeda Kuisiene Carlo Parini Donaldas Citavicius |
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Institution: | (1) Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21/27, 03101 Vilnius, Lithuania;(2) Department of Food Science and Microbiology, Industrial Microbiology Section, Faculty of Agriculture, University of Milan, Via Celoria 2, 20133 Milan, Italy |
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Abstract: | The complete nucleotide sequence (62.8 kb) of pGS18, the largest sequenced plasmid to date from the species Geobacillus stearothermophilus, was determined. Computational analysis of sequence data revealed 65 putative open reading frames (ORFs); 38 were carried
on one strand and 27 were carried on the other. These ORFs comprised 84.1% of the pGS18 sequence. Twenty-five ORFs (38.4%)
were assigned to putative functions; four ORFs (6.2%) were annotated as pseudogenes. The amino acid sequences obtained from
29 ORFs (44.6%) had the highest similarity to hypothetical proteins of the other microorganisms, and seven (10.8%) had no
significant similarity to any genes present in the current open databases. Plasmid replication region, strongly resembling
that of the theta-type replicon, and genes encoding three different plasmid maintenance systems were identified, and a putative
discontinuous transfer region was localized. In addition, we also found several mobile genetic elements and genes, responsible
for DNA repair, distributed along the whole sequence of pGS18. The alignment of pGS18 with two other large indigenous plasmids
of the genus Geobacillus highlighted the presence of well-conserved segments and has provided a framework that can be exploited to formulate hypotheses
concerning the molecular evolution of these three plasmids. |
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Keywords: | Geobacillus stearothermophilus Plasmid Complete nucleotide sequence Comparison |
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