荞麦组织培养及高频植株再生体系的建立

通过对荞麦(Fagopyrum esculentum Moench)不同外植体、不同激素配比的比较研究,建立了荞麦离体培养高效植株再生体系。荞麦子叶切段在含2．0 mg/L 2,4-D和1．0 mg/L 6-BA的MS培养基上愈伤组织诱导率为89．6％,而下胚轴切段在含2．0 mg/L 2,4-D和1．0-2．0 mg/L 6-BA MS培养基上愈伤组织诱导率高达100％。在2．0 mg／L 6-BA、0．1 mg,L IAA和1 mg／L KT的MS培养基上通过愈伤组织间接分化或外植体直接分化形成不定芽。来自子叶和下胚轴的愈伤组织的分化率分别为42．5％和73．6％,下胚轴的分化率明显高于子叶。将生长状态良好的不定芽转至含1．0 mg/L IBA和0．5mg/L NAA的1/2 MS培养基上生根,生根率达到100％。再生植株移栽到盆土中,成活率达91．6％,并且生长状态和特征均表现正常。

TISSUE CULTURE AND HIGH-FREQUENCY PLANT REGENERATION OF BUCKWHEAT (FAGOPYRUM ESCULENTUM MOENCH)

Different explants and compounding proportions of different hormones were comparatively studied in tissue culture of Fagopyrum esculentum Moench and thereafter an efficient plant -regeneration system was established by in vitro F.esculentum Moench culture. On MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA,89.6% cotyledon segments could be induced to produce calli; however,on MS medium containing 2.0 mg/L 2,4-D and 1.0-2.0 mg/L 6-BA,the induction rate of hypocotyl segments could reach as high as 100%. On MS medium containing 2.0 mg/L 6-BA,0.1 mg/L IAA and 1 mg/L KT,adventitious buds could be regenerated indirectly from calli or directly from explants,the differentiation rates of cotyledon-derived calli and hypocotyl-derived calli were 42.5% and 73.6% respectively; the calli coming from hypocotyl segments differentiated evidently at a higher rate than the calli originated from cotyledon segments. Well-grown adventitious buds were inoculated on 1/2 MS medium containing 1.0 mg/L IBA and 0.5mg/L NAA,100% rooting frequency was obtained. Plantlets grew well and appeared normal with no mortality after being transplanted to soil. Moreover,the survival rate of plantlets reached 91.6%.