Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection |
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| Authors: | Kaiyin Min Bin Ji Min Zhao Tiefeng Ji Bin Chen Xuedong Fang Qingjie Ma |
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| Institution: | 1 Department of Nuclear Medicine, China-Japan Union Hospital of Jilin University, Changchun 130033, China, ; 2 Department of General Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China, ; The University of Hong Kong, HONG KONG, |
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| Abstract: | Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, noninvasive visualization and quantification of MMP in vivo are of great interest in basic research and clinical application for breast cancer early diagnosis. Herein, we developed a 99mTc labeled membrane type I matrix metalloproteinase (MT1-MMP) specific binding peptide, 99mTc]-(HYNIC-AF7p)(tricine)(TPPTS), for in vivo detection of MDA-MB-231 breast tumor by single photon emission computed tomography (SPECT). 99mTc]-(HYNIC-AF7p)(tricine)(TPPTS) demonstrated nice biostability and high MT1-MMP binding affinity in vitro and in vivo. Tumor-to-muscle ratio was found to reach to the highest (4.17±0.49) at 2 hour after intravenously administration of 99mTc]-(HYNIC-AF7P)(tricine)(TPPTS) into MDA-MB-231 tumor bearing mice. Overall, 99mTc]-(HYNIC-AF7P)(tricine)(TPPTS) demonstrated great potential for MT1-MMP targeted detection in vivo and it would be a promising molecular imaging probe that are probably beneficial to breast cancer early diagnoses. |
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