Cloning and sequencing of cDNA for mouse liver metallothionein-I |
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Authors: | M Mbikay I B Maiti J P Thirion |
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Affiliation: | Départment de Microbiologie, Faculté de Médecine, Centre Hospitalier Universitaire, Université de Sherbrooke, Sherbrooke, P.Q., Canada J1H 5N4 |
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Abstract: | Metallothionein mRNA was purified from liver of mice injected with cadmium. The corresponding double-stranded cDNA was prepared and inserted into the PstI site of plasmid pBR322. The resulting recombinant DNA was used to transform the RR1 strain of . Clones resistant to tetracycline and sensitive to ampicillin were screened for the presence of metallothionein-specific restriction fragments in their plasmids. One plasmid, called M135, contains a cDNA insert covering the entire length of the mRNA for mouse liver metallothionein-I, except for the first 18 bases at the 5′ end. |
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Keywords: | MT metallothioneins bp base pair tetracycline resistant Ap ampicillin sensitive cadmium resistant |
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