Two-Dimensional Crystalline Array Formation of Glucuronide Transporter from Escherichia coli by the Use of Polystyrene Beads for Detergent Removal |
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| Authors: | Noriyuki Ishii |
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| Institution: | 1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central-6 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan
2. Life Science Division, Lawrence Berkeley National Laboratory, Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA
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| Abstract: | n-Dodecyl-β-d-maltoside solubilized glucuronide transporter (GusB), the product of gusB gene from Escherichia coli, was treated with Bio-Beads as an agent for removing the detergent from a micellar solution under suitable combination with dimyristoylphosphatidylcholine. Optimizing conditions led to a two-dimensional crystalline array formation of GusB. The crystalline arrays appear to have a hexagonal lattice with layer group P6, the unit cell dimensions of a = b = 13.8 nm and γ = 120°. Each stain-protruding periodic unit showed approximately 11.8 ± 0.3 nm in a diameter in the inverse Fourier-filtered image to have formed with pentameric GusB (5 × 49.7 kDa). |
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