Protein deficient chloroplast ribosomes in a yellow mutant of Chlamydomonas reinhardii |
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| Authors: | I. Gyurján N. P. Yurina M. S. Turischeva M. S. Odintsova N. N. Alexandrova |
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| Affiliation: | (1) Department of Genetics, Eötvös Loránd University, Budapest, Hungary;(2) Laboratory of Evolutionary Biochemistry and Subcellular Plant Organelles, A.N. Bakh Institute of Biochemistry, Moscow, USSR;(3) Department of Genetics, Leningrad State University, Leningrad, USSR |
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| Abstract: | Ribosomes and ribosomal proteins from wild-type and a yellow mutant of Chlamydomonas reinhardii were analyzed and compared by two-dimensional gel electrophoresis. The mixothrophically grown yellow-76 mutant differs from wild-type cells in lowered chlorophyll content and photosynthetic activity per chlorophyll unit. The latter is connected with the decreased activity of the ribulose-I,5-diphosphate-carboxylase enzyme. Analytical ultracentrifugation of cell extracts shows a normal amount of free 70S ribosomes and 50S subunit in the mutant cells. Two-dimensional gel electrophoresis shows considerable alterations in the protein composition of the 70S ribosomes of the mutant. Two proteins are absent from the electrophoretograms of the yellow-76 mutant, and seven proteins are present in reduced amounts. The genetical analysis shows a Mendelian pattern of inheritance, indicating that protein alterations presumably are localized in nuclear DNA.Abbreviation MNNG N-methyl-N -nitro-N-nitrosoguanidine |
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| Keywords: | Chlamydomonas Chloroplast mutant Mutant (chloroplast ribosomes) Photosynthesis Ribosomes Ribulosebiophosphate carboxylase |
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