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Plant-derived EpCAM antigen induces protective anti-cancer response |
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| Authors: | Robert Brodzik Sergei Spitsin Max Golovkin Katarzyna Bandurska Carla Portocarrero Monika Okulicz Zenon Steplewski Hilary Koprowski |
| Affiliation: | (1) Biotechnology Foundation Laboratories, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA 19107, USA;(2) Present address: Department of Animal Physiology and Biochemistry, August Cieszkowski Agricultural University, Wolynska 35, 60-637 Poznan, Poland |
| Abstract: | Immunotherapy holds great promise for treatment of infectious and malignant diseases and might help to prevent the occurrence and recurrence of cancer. We produced a plant-derived tumor-associated colorectal cancer antigen EpCAM (pGA733) at high yields using two modern plant expression systems. The full antigenic domain of EpCAM was efficiently purified to confirm its antigenic and immunogenic properties as compared to those of the antigen expressed in the baculovirus system (bGA733). Recombinant plant-derived antigen induced a humoral immune response in BALB/c mice. Sera from those mice efficiently inhibited the growth of SW948 colorectal carcinoma cells xenografted in nude mice, as compared to the EpCAM-specific mAb CO17-1A. Our results support the feasibility of producing anti-cancer recombinant vaccines using plant expression systems. |
| Keywords: | Plant biotechnology Colorectal cancer Recombinant subunit vaccine |
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