Biophysical methods for biochip analysis. Use of wide-field digital fluorescence microscopy

This paper discusses the development of biophysical methods for biochip analysis. A scheme and construction of a biochip analyzer based on wide-field digital fluorescence microscopy are described. The analyzer is designed to register images of biological microchips labeled with fluorescent dyes. The device developed is useful for high-sensitivity throughput recording of analyses with biochips after interaction of immobilized probes with fluorescently labeled sample molecules as well as it provides a higher rate of the analysis compared with laser scanning devices. With this analyzer, the scope where biological microchips can be applied becomes wider, development of new protocols of the analyses is possible and standard analyses run faster with the use of biochips, the expenses for performing routine analyses can be reduced.