|
|||||
|
|
Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers |
|
| Authors: | J Rouppe van der Voort P Wolters R Folkertsma R Hutten P van Zandvoort H Vinke K Kanyuka A Bendahmane E Jacobsen R Janssen J Bakker |
| Institution: | (1) The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, P.O. Box 8123, 6700 ES Wageningen, The Netherlands Fax: +31 317 484 254 E-mail: Jeroen.RouppevanderVoort@medew.nema.wau.nl, NL;(2) The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AJ Wageningen, The Netherlands, NL;(3) The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The Netherlands, NL;(4) The Sainsbury Laboratory, Norwich Research Park, Colney, Norwich NR4 7UH, UK, GB |
| Abstract: | The nematode resistance locus Gpa2 was mapped on chromosome 12 of potato using information on the genomic positions of 733 known AFLP markers. The minimum number of AFLP primer combinations required to map Gpa2 was three. This demonstrates that a reference collection of potato AFLP markers may be a valuable tool for mapping studies in potato. By use of RFLP probes, Gpa2 was more precisely mapped at the distal end of chromosome 12. Gpa2 confers resistance to a distinct group of populations of the potato cyst nematode Globodera pallida and originates from the same potato accession as locus H1, conferring resistance to pathotype Ro1 of G. rostochiensis. This study shows that these two nematode resistance loci are unlinked and that Gpa2 is linked to the Rx1 locus conferring resistance to potato virus X. The efficiency of AFLPs for genetic mapping of a highly heterozygous crop like potato is discussed and compared with the RFLP technique. Received: 24 February 1997/Accepted: 2 May 1997 |
| Keywords: | Gene-for-gene Globodera pallida G rostochiensis Potato Virus X Solanum tuberosum ssp andigena CPC 1673 |
| 本文献已被 SpringerLink 等数据库收录！ | |