Retroviral-mediated gene transfer as an effective tool for the in vitro genetic transformation of chicken embryonic cells and production of transgenic chickens |
| |
| Authors: | N. A. Volkova N. A. Zinovieva L. A. Volkova L. K. Ernst |
| |
| Affiliation: | (1) Russian State Institute of Animal Breeding, Russian Academy of Agricultural Sciences, Dubrovitsy Moscow oblast, 142132, Russia;(2) Russian Academy of Agricultural Sciences, Moscow, 117218, Russia |
| |
| Abstract: | The data on the in vitro and in vivo (into embryonic disk) retroviral-mediated transfer of genetic information into chicken embryonic cells are presented. The estimated transformation frequency of the cultured target cells constituted 8 × 10?4 to 5 × 10?3. A transgenic rooster, carrying recombinant DNA in blood, heart, liver, and intestine cells, was obtained. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
