Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin |
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Authors: | P I Payne L M Holt C N Law |
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Institution: | (1) Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, England |
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Abstract: | Summary The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although
each variety contained, with only a few exceptions, between 3 and 5 subunits. Further inter-varietal substitution lines to
those already described (Payne et al. 1980) were analysed and the results indicate that all the HMW subunits are controlled
by the homoeologous group 1 chromosomes. All hexaploid varieties studied except ‘NapHal’ contained two major subunits controlled
by chromosome 1D. Their genes were shown to be tightly linked genetically for only four different types of banding patterns
were observed. The nominal molecular weights determined after fractionation in 10% polyacrylamide gels were between 110,000
and 115,000 for the larger of the two subunits and between 82,000 and 84,000 for the smaller. One quarter of the varieties
contained only one major HMW subunit controlled by chromosome 1B whereas the rest had two. The chromosome 1B subunits were
the most varied and nine different banding patterns were detected. All the subunits had mobilities which were intermediate
between those of the two chromosome 1D-controlled subunits. Only two types of HMW subunit controlled by chromosome 1A were
detected in all the varieties examined; a single variety never contained both of these subunits and 40% of varieties contained
neither. The chromosome 1A-controlled subunits had slightly slower mobilities in 10% gels than the largest HMW subunit controlled
by chromosome 1D. About 100 single grains were analysed from each of five different crosses of the type (F1 of variety A × variety B) × variety C. The results indicate that the genes on chromosome 1B which control the synthesis of
subunits 6, 7, 13, 14 and 17 are allelic, as are the genes of the chromosome 1A-controlled subunits, 1 and 2. |
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Keywords: | Glutenin Triticum Genetics SDS Polyacrylamide-gel-electrophoresis |
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