Studies on triacylglycerol ester hydrolase from bat adipose tissue |
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Authors: | Subhash S Patil Chanda K Bhandari Vijay A Sawant |
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Institution: | (1) Animal Physiology Laboratory, Zoology Department, Shivaji University, 416 004 Kolhapur |
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Abstract: | Triacylglycerol ester hydrolase was isolated from bat adipose tissue and characterized. The partially purified enzyme had
pH optimum of 8.6 and a Km value of 0.6 mM. The enzyme was denaturated upon freezing and thawing, which was prevented by 25% glycerol. The enzyme was
activated by EDTA and NaCl, while it was inhibited by serum and bovine serum albumin. Heparin, sodium fluoride and diisopropyl
fluorophosphate had no effect on triacylglycerol ester hydrolase activity. It hydrolyzed triglycerides partially. Triacylglycerol
ester hydrolase lost its activity during delipidation but it was reactivated by endogenous lipids and phospholipids, viz.
phosphatidyl ethanolamine, phosphatidyl choline and sphingomyelin. The enzyme shows kinetic properties altogether different
from lipoprotein lipase and hormone sensitive lipase |
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Keywords: | Bat adipose tissue triacylglycerol ester hydrolase bovine serum albumin phospholipids |
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