Functional reconstitution of rat ovarian LH/hCG receptor into proteoliposomes |
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Authors: | Jaroslav Kolena |
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Affiliation: | Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava. |
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Abstract: | Rat ovarian membrane LH/hCG receptor was solubilized in various detergents and reconstituted into proteoliposomes. Upon removal of sodium cholate by active absorption on Bio-Beads SM-2, the functional interaction between receptor and adenylate cyclase was restored. Adenylate cyclase was stimulated by hCG, HCG+GTP or GppNHp and NaF. Reconstituted proteoliposomes bound more 125I-hCG (528 fmol/mg protein) than membrane-bound receptors (384 fmol/mg protein). There was no difference, however, in the relative affinity of reconstituted receptor preparations for hCG. |
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Keywords: | Reconstitution Hormone receptor Sodium cholate Adenylate cyclase (Rat ovary) |
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