Metabolite profiles of Stachybotrys isolates from water-damaged buildings and their induction of inflammatory mediators and cytotoxicity in macrophages |
| |
Authors: | Nielsen Kristian Fog Huttunen Kati Hyvärinen Anne Andersen Birgitte Jarvis Bruce B Hirvonen Maija-Riitta |
| |
Affiliation: | The Mycology Group, BioCentrum-DTU, Technical University of Denmark, Kgs. Lyngby. kristian.f.nielsen@biocentrum.dtu.dk |
| |
Abstract: | The metabolite profiles of 20 Stachybotrys spp. isolates from Finnish water-damaged buildings were compared with their biological activities. Effects of purified compounds on cytotoxicity and production of inflammatory mediators such as nitric oxide, IL-6 and TNFalpha in murine RAW264.7 macrophage cells were studied. The 11 isolates belonging to the satratoxin-producing chemotype were highly cytotoxic to the macrophages. The isolates inducing inflammatory mediators all belonged to the atranone-producing chemotype, but pure atranones B, and D did not elicit a response in the bioassay. Altogether, cytotoxicity of Stachybotrys sp. isolates appear to be related to satratoxin production whereas the specific component inducing inflammatory responses in atranone-producing isolates remains obscure. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|