Parallel measurements of heat production and thermogenin content in brown fat cells during cold acclimation of rats |
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| Authors: | Erik Steen Hansen Jens Knudsen |
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| Institution: | (1) Institute of Physiology, Odense University, Denmark;(2) Institute of Biochemistry, Odense University, Denmark |
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| Abstract: | The maximum thermogenic capacity of brown fat cells from control and cold acclimated rats was measured using a continuous-flow microcalorimetric system, The content of the 32.000 D, brown fat specific protein, thermogenin, was measured in the cells used for heat production measurements by competitive ELISA. The ratio between the maximal thermogenic capacity and the amount ofthermogenin for control and cold acclimated rats was compared. It was found that the ratio between the two parameters decreased during cold acclimation due to a decrease in maximal thermogenic capacity and an increase in the amount ofthermogenin, indicating regulation of heat production either at thermogenin or receptor level. |
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| Keywords: | brown fat cells heat production and thermogenin |
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