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Isolation and analysis of recombinant DNA molecules containing yeast DNA. |
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| Authors: | T D Petes J R Broach P C Wensink L M Hereford G R Fink D Botstein |
| Affiliation: | 1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA;2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA;3. Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02154, USA;4. Department of Genetics, Development and Physiology, Cornell University, Ithaca, NY, USA;5. Department of Microbiology, University of Chicago, 920 E. 58th Street, Chicago, IL 60637, U.S.A. |
| Abstract: | 2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species. |
| Keywords: | pMB9 plasmid yeast nuclear DNA genes for tRNA and rRNA abundant mRNA |
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