Iron-dependent vs. iron-independent cold-induced injury to cultured rat hepatocytes: a comparative study in physiological media and organ preservation solutions |
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Authors: | Rauen Ursula Kerkweg Uta de Groot Herbert |
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Institution: | Institut für Physiologische Chemie, Universit?tsklinikum, Hufelandstr. 55, 45122 Essen, Germany. ursula.rauen@uni-duisburg-essen.de |
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Abstract: | We previously described the entity of cold-induced apoptosis to rat hepatocytes and characterized its major, iron-dependent pathway. However, after cold incubation in some solutions, e.g. cell culture medium, hepatocytes show an additional, yet uncharacterized component of cold-induced injury. We here assessed the effects of organ preservation solutions on both components of cold-induced injury and tried to further characterize the iron-independent component. None of the preservation solutions (University of Wisconsin, histidine-tryptophan-ketoglutarate, Euro-Collins, histidine-lactobionate, sodium-lactobionate-sucrose and Celsior solutions) provided significant protection against cold-induced cell injury (LDH release after 24-h cold incubation/3h rewarming >65% for all solutions); three solutions even enhanced cold-induced injury. However, when the predominant iron-dependent mechanism was eliminated by the addition of iron chelators, all preservation solutions yielded hepatocyte protection that was clearly superior to the one obtainable in cell culture medium or Krebs-Henseleit buffer with iron chelators (LDH release after 24-h cold incubation/3h rewarming
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Keywords: | Hypothermia University of Wisconsin solution Histidine-tryptophan-ketoglutarate solution Celsior solution Euro-Collins solution Sodium-lactobionate-sucrose solution Histidine-lactobionate solution Cold-induced apoptosis Chloride Sodium |
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