Glial fibrillary acidic protein and differentiation of neonatal rat pituicytes in vitro |
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Authors: | Halina Weinrander Anna Kowalska |
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Affiliation: | (1) Department of Neuropathology, Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland;(2) Department of Neuropathology, Medical Research Centre, Polish Academy of Sciences, 3 Dworkowa Street, 00-784 Warsaw, Poland |
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Abstract: | Summary The appearance and intracellular localisation of glial fibrillary acidic protein (GFAP) in pituicytes in neural lobe cultures of newborn rats aged 7 to 30 days were investigated by use of the indirect immunofluorescence method. GFAP-immunoreactive cells were observed mostly in the outgrowth zone. GFAP was localised in the perikaryal cytoplasm as well as in pituicyte processes. GFAP-positive pituicytes showed considerable morphological polymorphism. The presence of GFAP — astrocytic marker — in pituicytes in vitro and the evident morphological similarity to cultured astrocytes suggest the astroglial character of these cells. |
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Keywords: | Glial fibrillary acidic protein (GFAP) Pituicytes, neonatal Development, ontogenetic Immunofluorescence Organ culture Rat |
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