Multiple shoot regeneration and tissue culture studies on Bacopa monnieri (L.) Pennell |
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Authors: | N Shrivastava M Rajani |
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Institution: | (1) Pharmacognosy and Phytochemistry Department, B.V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej – Gandhinagar Highway, Thaltej, Ahmedabad – 380 054, India Fax: +091-79-7450449, IN |
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Abstract: | Adventitious shoot buds were induced from leaf and stem explants of Bacopa monnieri on Murashige and Skoog medium supplemented with benzyladenine or kinetin. The source of the explants as well as different
gelling agents in the medium were found to influence shoot induction and eventual shoot growth. The best response was obtained
in leaf explants taken from shoot cultures grown in medium supplemented with 2 μM benzyladenine and gelled with 0.2% gelrite. A transverse section of the leaf explant incubated in this medium showed several
shoot primordia emerging from the leaf surface. This system exhibited a potential for repeated harvesting of the shoots from
the original leaf explant as the latter continued to expand and regenerate new shoots, upon repeated periodical subculturing
onto fresh medium. However, the callusing response of the plant was very low. Qualitative TLC studies of the regenerated shoots
revealed a phytochemical profile similar to that of the field grown-plants.
Received: 20 March 1998 / Revision received: 1 December 1998 / Accepted: 12 December 1998 |
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Keywords: | Callus Explant source Multiple shoot-bud induction Gelrite Shoot cultures |
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