Vanadate inhibition of sarcoplasmic reticulum Ca2+-ATPase and other ATPases. |
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Authors: | S G O'Neal D B Rhoads E Racker |
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Institution: | Section of Biochemistry, Molecular & Cell Biology Cornell University, Ithaca, New York 14853 USA |
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Abstract: | Vanadate is a potent inhibitor of the Ca2+-ATPase activity of sarcoplasmic reticulum in the presence of A-23187. The purified enzyme is sensitive to vanadate even in the absence of the ionophore. Ca2+ and norepinephrine protect the enzyme against inhibition of vanadate. The nonspecificity of vanadate is emphasized by the finding of inhibition of several other ATPases including the Ca2+Mg2+-ATPases of the ascites and human red cell plasma membranes, Mg2+-ATPase of the ascites plasma membrane, and the K+-ATPases of and hog gastric mucosal cell membranes. The ascites plasma membrane Ca2+-ATPase (an ecto ATPase) and mitochondrial ATPase are not inhibited by vanadate. |
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Keywords: | SR sarcoplasmic reticulum |
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