Lactic acid production from sugar-cane juice by a newly isolated Lactobacillus sp. |
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Authors: | Walaiporn Timbuntam Klanarong Sriroth Yutaka Tokiwa |
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Institution: | (1) Kasetsart Agricultural and Agro-Industrial Improvement Institute, Kasetsart University, Bangkok, 10900, Thailand;(2) Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand;(3) National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan |
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Abstract: | A newly isolated sucrose-tolerant, lactic acid bacterium, Lactobacillus sp. strain FCP2, was grown on sugar-cane juice (125 g sucrose l−1, 8 g glucose l−1 and 6 g fructose l−1) for 5 days and produced 104 g lactic acid l−1 with 90% yield. A higher yield (96%) and productivity (2.8 g l−1 h−1) were obtained when strain FCP2 was cultured on 3% w/v (25 g sucrose l−1, 2 g glucose l−1 and 1 g fructose l−1) sugar-cane juice for 10 h. Various cheap nitrogen sources such as silk worm larvae, beer yeast autolysate and shrimp wastes
were also used as a substitute to yeast extract. |
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Keywords: | Lactic acid Lactobacillus sp Nitrogen sources Sugar-cane juice |
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