Thermolabile analogue proteins and the entry of HeLa and CHO cells into division |
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Authors: | D N Wheatley J Y Henderson |
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Affiliation: | Department of Pathology, University Medical School, Foresterhill, Aberdeen AB9 2ZD, Scotland |
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Abstract: | Entry of HeLa and CHO-10 cells into mitosis can be inhibited by incorporation of p-fluorophenylalanine at certain temperatures, 37 °C for the former cell type and 39.5 °C for the latter. At lower temperatures, 32 °C in the former and 37 °C in the latter, the analogue does not inhibit entry of cells into mitosis. The possibility that the analogue is not incorporated at the permissive temperatures has been ruled out; indeed incorporation is relatively greater at the permissive temperatures. The results suggest that the physiological properties of analogue protein molecules differ depending on the temperature at which they are synthesized; the higher the temperature the more likely they are to malfunction. |
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