Structure of the extracellular gelling polysaccharide produced by Enterobacter (NCIB 11870) species |
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| Authors: | M A O'Neill V J Morris R R Selvendran I W Sutherland I T Taylor |
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| Institution: | 1. Burdock Group, 859 Outer Road, Orlando, FL 32814, USA;2. Product Safety Laboratories, 2394 Highway 130, Dayton, NJ 08810, USA;1. Department of Pathology and Laboratory Medicine, McGovern Medical School at UTHealth, Houston, TX 77030, USA;2. Center for Infectious Disease, Department of Molecular Biosciences, Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA;3. Department of Biology, University of Utah, Salt Lake City, UT 84112, USA;1. Department of Pediatrics, Columbia University Irving Medical Center, New York, NY 10032, USA;2. Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA;3. Proteomics and Macromolecular Crystallography Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA;4. Department of Microbial Pathogenesis, University of Maryland, Baltimore, Baltimore, MD 21201, USA;1. Physikalische Biochemie, Universität Potsdam, Potsdam, Germany;2. Department Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany;3. Crystallography, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany;4. Physikalische Zellbiochemie, Universität Potsdam, Potsdam, Germany;5. Institut für Chemie und Biochemie, Freie Universität, Berlin, Germany |
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| Abstract: | The gelling polysaccharide produced by a species of Enterobacter (NCIB 11870) contains L-fucose, D-glucose, and D-glucuronic acid in the ratios 1:2:1. Analysis of the methylated and methylated, carboxyl-reduced polysaccharide revealed terminal non-reducing glucose, (1----3)-linked fucose, (1----3,1----4)-linked glucose, and (1----4)-linked glucuronic acid in the ratios 1:1:1.2:0.8. From the results of Smith degradation of the polysaccharide and spectroscopic studies of the acidic tetra- and octa-saccharides produced by bacteriophage-induced enzymic depolymerization of the polysaccharide, the following tetrasaccharide repeating-unit is proposed. (Formula: see text). This repeating-unit is identical to that of the capsular polysaccharide produced by Klebsiella aerogenes serotype K54 except for the absence of O-acetyl groups. The effects of the O-acetyl groups on the secondary structure and rheological properties of these polysaccharides are discussed. |
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