Characterization of sucrose–glutamic acid Maillard products (SGMPs) degrading bacteria and their metabolites |
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| Authors: | Ram Chandra Ram Naresh Bharagava Vibhuti Rai Shio Kumar Singh |
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| Affiliation: | aEnvironmental Microbiology Section, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M.G. Marg, Lucknow 226001, Uttar Pradesh, India;bSchool of Studies in Life Sciences, Pt. Ravishankar Shukla University, Raipur 492010, Chhattisgarh, India;cPharmacokinetics and Metabolism Division, Central Drug Research Institute (CSIR), Chatter Manzil, Lucknow 226001, Uttar Pradesh, India |
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| Abstract: | Two aerobic bacteria RNBS1 and RNBS3 capable to degrade and utilize sucrose–glutamic acid Maillard products (SGMPs) as carbon, nitrogen and energy source were isolated and characterized as Alcaligenes faecalis (DQ659619) and Bacillus cereus (DQ659620) respectively by 16S rRNA gene sequence analysis. In present study, mixed bacterial culture was found more effective compared to axenic culture RNBS1 and RNBS3 decolourizing 73.79%, 66.80% and 62.56% SGMPs, respectively. The SGMPs catabolizing enzyme was characterized as manganese dependent peroxidase (MnP) by SDS–PAGE yielding a single band of 43 KDa. Further, the LC-MS–MS and other spectrophotometric analysis have revealed that most of the SGMPs detected in control were diminished from bacteria treated samples. The disappearance of SGMPs from bacteria treated samples could be related with the degradation of SGMPs. |
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| Keywords: | Maillard product Degradation Alcaligenes faecalis Bacillus cereus Metabolite characterization |
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