| 肝素的定量法 |
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| 引用本文： | 张昌敬,黎义和,李贤柏.肝素的定量法[J].生物化学与生物物理进展,1994,21(4):372-373. |
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| 作者姓名： | 张昌敬 黎义和 李贤柏 |
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| 作者单位： | 重庆师范学院生化教研室;重庆师范学院生化教研室;重庆师范学院生化教研室 |
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| 基金项目： | 重庆市科委按拔款资助重点项目. |
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| 摘 要： | 根据核糖核酸酶水解核糖核酸时，在300nm波长处光吸收值下降的速率被肝素抑制之特点，用已知量肝素对抑制程度进行定量，制得标准检量线，从而测得未知量的肝素含量．此法简捷方便，一次能测定多个样品．
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| 关 键 词： | 核糖核酸酶 核糖核酸 猪肺源肝素 光吸收值 |
| 收稿时间： | 5/5/1993 12:00:00 AM |
| 修稿时间： | 1993/6/20 0:00:00 |
Quantitative Method of Heparin |
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| Zhang Changjing,Li Yihe and Li Xianbai.Quantitative Method of Heparin[J].Progress In Biochemistry and Biophysics,1994,21(4):372-373. |
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| Authors: | Zhang Changjing Li Yihe and Li Xianbai |
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| Institution: | Department of Biochemistry, Chcogqing Teachers Training College;Department of Biochemistry, Chcogqing Teachers Training College;Department of Biochemistry, Chcogqing Teachers Training College |
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| Abstract: | It is known that the dropping rate of optical absorbing value at 300 nm wave length when ribonucleic acid is hydrolysed with ribonuclease is inhibited quantitatively by heparin. Upon this fact. a standard measuring line is plotted when the inhibition is quantitated by known quantity standard heparin. Any unknown can be determined conveniently by comparing with this standard. |
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| Keywords: | ribonuclease ribonucleic acid heparin from hog lung optical absorbing value |
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