Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression |
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| Authors: | Christelle Alexa Garcia Perez Shungo Adachi Quang Dang Nong Nikko Adhitama Tomoaki Matsuura Toru Natsume Tadashi Wada Yasuhiko Kato Hajime Watanabe |
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| Affiliation: | 1. Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan ; 2. Cellular and Molecular Biotechnology Research Institute (CMB), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan ; The University of Manchester Manchester Institute of Biotechnology, UNITED KINGDOM |
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| Abstract: | Long noncoding RNAs (lncRNAs) are vastly transcribed and extensively studied but lncRNAs overlapping with the sense orientation of mRNA have been poorly studied. We analyzed the lncRNA DAPALR overlapping with the 5´ UTR of the Doublesex1 (Dsx1), the male determining gene in Daphnia magna. By affinity purification, we identified an RNA binding protein, Shep as a DAPALR binding protein. Shep also binds to Dsx1 5´ UTR by recognizing the overlapping sequence and suppresses translation of the mRNA. In vitro and in vivo analyses indicated that DAPALR increased Dsx1 translation efficiency by sequestration of Shep. This regulation was impaired when the Shep binding site in DAPALR was deleted. These results suggest that Shep suppresses the unintentional translation of Dsx1 by setting a threshold; and when the sense lncRNA DAPALR is expressed, DAPALR cancels the suppression caused by Shep. This mechanism may be important to show dimorphic gene expressions such as sex determination and it may account for the binary expression in various developmental processes. |
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