Bioinsecticidal activity of conidia and dry mycelium preparations of two isolates of Beauveria bassiana against the sugarcane borer Diatraea saccharalis |
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| Institution: | 1. Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI, Facultad de Ciencias Exactas, 47 y 115, 1900, La Plata, Argentina;2. Instituto de Microbiolog??a y Zoolog??a Agr??cola, IMYZA-CICA-INTA Castelar, C.C. 25, 1712, Castelar, Argentina;1. USDA-ARS U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945, USA;2. Citrus Center, Texas A&M University-Kingsville, Weslaco, TX 78596, USA;3. USDA-ARS Hayden Bee Research Center, Tucson, AZ 85719, USA;4. USDA-ARS National Center for Agricultural Utilization Research, Peoria, IL 61604, USA;5. USDA-APHIS Center for Plant Health Science and Technology, Mission, TX 78451, USA;6. University of Florida, Institute of Food and Agricultural Sciences, Indian River Research and Education Center, Fort Pierce, FL 34945, USA;7. USDA-ARS Southern Horticulture Research Laboratory, Poplarville, MS 39470, USA;1. Department of Agriculture, Payam Noor University (PNU), P.O.BOX 19395-3697 Tehran, Iran;2. Department of Sustainble Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy;1. Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement / Biotechnology Research Center, China Three Gorges University, Yichang 443000, China;2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;1. School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China;2. Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Two isolates of Beauveria bassiana (Bb1 and Bb5 strains) were grown in solid-state and submerged cultures (SSC and SbC, respectively) in order to obtain conidia and dry mycelium preparations. The samples obtained at laboratory scale were tested as alternatives for their further use as mycoinsecticides to control the sugarcane borer Diatraea saccharalis. The spore yields obtained in SSC indicated that the Bb5 strain was able to produce around three times more conidia per gram of initial dry matter (3.7×1010) than the Bb1 strain (1.3×1010) in a solid medium composed of wheat bran and bran husk. Under this culture conditions, the former showed not only satisfactory spore yields, but also a higher bioinsecticidal activity than the latter. Laboratory bioassays carried out with conidia of both strains against D. saccharalis larvae indicated that the Bb5 strain promotes an insect mortality of 82.5% whereas only 21.3% was observed with the Bb1 strain preparations. Concerning the samples obtained in SbC, in which a culture medium based on glucose and yeast extract was employed, it is interesting to point out that although both strains showed similar behaviors with yields of approximately 1.50×1010 conidia per gram of dry mycelium, both preparations failed in their bioinsecticidal activities. Despite satisfactory yields, bioinsecticidal activity of the Bb1 and Bb5 strains dropped drastically showing a larvae mortality below 2%. |
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