Mineralization and solubilization of synthetic lignin by manganese peroxidases from Nematoloma frowardii and Phlebia radiata |
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| Affiliation: | 1. Friedrich Schiller University of Jena, Institute of Microbiology, Department Technical Microbiology, Philosophenweg 12, D-07743, Jena, Germany;2. Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1 (Viikinkaari 9), University of Helsinki, 00014, Helsinki, Finland;3. Department of Chemistry, P.O. Box 55, University of Helsinki, 00014, Helsinki, Finland;1. Institute of Inorganic Chemistry, Technology and Materials, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-81237 Bratislava, Slovakia;2. Institute of Inorganic Chemistry of the AS CR, v.v.i., Husinec-Řež č.p. 1001, CZ-25068 Řež, Czech Republic;3. Material Science, Darmstadt University of Technology, D-64287 Darmstadt, Germany;4. Department of General and Inorganic Chemistry, Faculty of Chemical Technology, University of Pardubice, CZ-53210 Pardubice, Czech Republic;5. Department of Chemistry, FPV, University of SS Cyril and Methodius, SK-91701 Trnava, Slovakia;1. Dpto. Automatica y Computacion, Universidad Publica de Navarra, 31006 Pamplona, Spain;2. KERMIT, Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University, Coupure links 653, 9000 Ghent, Belgium;3. Laboratory of Wood Technology, Department of Forest and Water Management, Ghent University, Coupure links 653, 9000 Ghent, Belgium;1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, Beijing, 100085, PR China;2. University of Chinese Academy of Sciences, Beijing, 100049, PR China;3. Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300071, PR China;4. Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331, USA;5. Joint Institute for Environmental Science, Research Center for Eco-Environmental Sciences and Hong Kong Baptist University, Beijing/Hong Kong, PR China;1. NERC Centre for Ecology & Hydrology, Bush Estate, Penicuik, Midlothian Scotland EH26 0QB, UK;2. Forest Research, Northern Research Station, Roslin, Midlothian EH25 9SY, UK;1. UFZ - Helmholtz Centre for Environmental Research, Department of Computational Landscape Ecology, Permoserstr. 15, 04318 Leipzig, Germany;2. Leuphana University Lüneburg, Faculty of Sustainability, Scharnhorststr. 1, 21355 Lüneburg, Germany;3. Leuphana University Lüneburg, Centre of Methods, Scharnhorststr. 1, 21335 Lüneburg, Germany;4. UFZ - Helmholtz Centre for Environmental Research, Department of Economics, Permoserstr. 15, 04318 Leipzig, Germany;5. UFZ - Helmholtz Centre for Environmental Research, Department of Community Ecology, Theodor-Lieser-Str. 4, 06120 Halle, Germany;6. Martin-Luther-University Halle-Wittenberg, Department of Biology/Geobotany and Botanical Garden, Am Kirchtor 1, 06108 Halle (Saale), Germany;7. University of Leipzig, Faculty of Biosciences, Pharmacy and Psychology, Institut of Biology – Molecular Evolution and Animal Systematics, Talstr. 33, 04103 Leipzig, Germany;8. Vilnius University, Faculty of Natural Sciences, Department of Zoology, M.K.Čiurlionio str. 21/27, LT-03101, Vilnius, Lithuania;9. University of Aveiro, Department of Social, Political and Territorial Sciences, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal;10. Martin-Luther-University Halle-Wittenberg, Institute of Geoscience and Geography, 06099 Halle (Saale), Germany |
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| Abstract: | Crude and purified manganese peroxidase from the white-rot fungi Nematoloma frowardii and Phlebia radiata catalyzed the partial depolymerization of a [14C-ring]labelled synthetic lignin into water-soluble fragments (30–50%). The in vitro depolymerization of the 14C-labelled lignin was accompanied by a release of 14CO2 ranging from 4 to 6%. Small quantities of the thiol mediator glutathione stimulated the depolymerization of lignin resulting in a mineralization and solubilization of up to 10 and 64%, respectively. Most of the water-soluble substances formed had molecular masses around 0.7 kDa, although a higher-molecular mass fraction was also detectable (>2 kDa). Photometric assays using 2,2′-azinobis(3-ethylbenzothiazolinesulphonate) as an indicator demonstrated that high levels of Mn(III), which were very probably responsible for the depolymerization and mineralization of the 14C-labelled lignin, were adjusted within the first 24 h of incubation. The manganese peroxidase catalyzed depolymerization process was not necessarily dependent on H2O2; also in the absence of the H2O2-generating system glucose/glucose oxidase, effective solubilization and mineralization of lignin dehydrogenation polymerizate occurred, due to the in part superoxide dismutase sensitive, ‘oxidase-like’ activity of MnP which probably produces radical species and peroxides from malonate. |
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