Peroxisomes exhibit compromised structure and matrix protein content in SARS-CoV-2-infected cells |
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| Authors: | Barbara Knoblach Ray Ishida Tom C Hobman Richard A Rachubinski |
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| Institution: | National Institutes of Health, NHLBI;aDepartment of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada;bDepartment of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada;cLi Ka Shing Institute of Virology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada |
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| Abstract: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel coronavirus that has triggered global health and economic crises. Here we report the effects of SARS-CoV-2 infection on peroxisomes of human cell lines Huh-7 and SK-N-SH. Peroxisomes undergo dramatic changes in morphology in SARS-CoV-2-infected cells. Rearrangement of peroxisomal membranes is followed by redistribution of peroxisomal matrix proteins to the cytosol, resulting in a dramatic decrease in the number of mature peroxisomes. The SARS-CoV-2 ORF14 protein was shown to interact physically with human PEX14, a peroxisomal membrane protein required for matrix protein import and peroxisome biogenesis. Given the important roles of peroxisomes in innate immunity, SARS-CoV-2 may directly target peroxisomes, resulting in loss of peroxisome structural integrity, matrix protein content and ability to function in antiviral signaling. |
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