Overexpression of DnaK from a halotolerant cyanobacterium Aphanothece halophytica acquires resistance to salt stress in transgenic tobacco plants |
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| Affiliation: | 1. School of Agriculture, Meijo University, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan;2. Department of Chemistry, Faculty of Science and Technology, Meijo University, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan;3. BioScience Center, School of Agricultural Science, Nagoya University, Chikusa-ku, Nagoya, Aichi 464-8601, Japan;4. Research Institute of Meijo University, Tenpaku-ku, Nagoya, Aichi 468-8502, Japan;1. Henan Key Laboratory of Nanocomposite and Application, Zhengzhou City Key Laboratory of Supercapacitor, Institute of Nanostructured Functional Materials, Huanghe Science and Technology College, Zhengzhou, 450006, Henan, China;2. Henan University of Engineering, College of Materials and Chemical Engineering, Zhengzhou, 450000, Henan, China;1. Istituto Zooprofilattico Sperimentale dell''Umbria e delle Marche, via Salvemini, 1, 06126 Perugia (PG), Italy;2. Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell’Universita’, 10-35 020 Legnaro (PD), Italy;3. Istituto Zooprofilattico Sperimentale della Lombardia e Emilia Romagna, sezione diagnostica di Pavia, Strada Campeggi 59/61, 27 100 Pavia (PV), Italy;4. Istituto Zooprofilattico Sperimentale della Sardegna, Via Duca degli Abruzzi 8, 7 100 Sassari (SS), Italy;5. Istituto Superiore di Sanità, Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Viale Regina Elena 299, 161, Roma, Italy;1. State Key Laboratory of Medical Molecular Biology, Department of Microbiology and Parasitology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, 100005, Beijing, China;2. Institute of Biochemistry and Molecular Biology, ZBMZ, University of Freiburg, Stefan-Meier-Straße 17, 79104, Freiburg im Breisgau, Germany;3. College of Life Sciences, Linyi University, Linyi, 276005, China;1. Department of Pharmaceutical Sciences, UNT System College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX 76107, United States;2. Department of Physiology, National Key Disciplines, Key Laboratory for Cellular Physiology of Ministry of Education, Shanxi Medical University, Taiyuan, 030001, China;1. Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa;2. Biotechnology Innovation Centre, Rhodes University, Grahamstown 6140, South Africa |
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| Abstract: | To test the role of the heat shock protein DnaK/Hsp70 in salt tolerance, transgenic plants of Nicotina tabacum cv Petit Havana SR1 were made with DnaK1 from a halotolerant cyanobacterium Aphanothece halophytica (A. halophytica) overexpressed in the cytosol. The growth rate and photosynthetic activities of the transgenic and control tobacco plants were similar under non-stressed conditions. The CO2 assimilation rate of the control plants decreased with increasing concentration of NaCl. After 3 days of treatment with 0.6 M NaCl, the CO2 fixation rate decreased to 40% of that in the non-stressed plants whereas its activity in the transgenic plants was about 85% of that in the non-stressed plants. Similar results were observed for the stomatal transpiration. The sodium contents in leaves of the control plants were significantly increased by salt stress whereas those in the transgenic plants remained at levels similar to those in the non-stressed plants. Total protein contents and ribulose 1,5-bis phosphate carboxygenase and oxygenase (RuBisCO) levels were decreased by salt stress in both the transgenic and control plants but the decrease was slight in the transgenic tobacco. All these data clearly indicate that the expression of DnaK1 from a halotolerant cyanobacterium A. halophytica improved the salt tolerance of the tobacco plant. |
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