New antioxidant mixture for long term stability of serotonin, dopamine and their metabolites in automated microbore liquid chromatography with dual electrochemical detection |
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Authors: | Katrien Thorré ,Miloslav Pravda,Sophie Sarre,Guy Ebinger,Yvette Michotte |
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Affiliation: | Katrien Thorré, Miloslav Pravda, Sophie Sarre, Guy Ebinger,Yvette Michotte, |
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Abstract: | An automated microbore liquid chromatographic assay with dual electrochemical detection is described for the determination of serotonin, dopamine and their metabolites, 5-hydroxyindoleacetic acid, 3,4-dihydroxyphenylacetic acid and homovanillic acid. Due to the chemical instability of the compounds, the addition of an antioxidant is required for automated analysis over a long period of time (e.g., 20 h). Therefore, the time stability of these substances was tested with different antioxidants. The stability for serotonin and 5-hydroxyindoleacetic acid was poor in acidic medium containing Na2EDTA but could greatly be improved by the addition of -cysteine and ascorbic acid. Using this assay, the neurotransmitters and their metabolites could easily be determined in microdialysates obtained from different rat brain areas. |
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Keywords: | Serotonin Dopamine 5-Hydroxyindoleacetic acid 3,4-Dihydroxyphenylacetic acid Homovanillic acid |
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