Cloning and expression of Staphylococcus epidermidis urease gene sequences in Staphylococcus carnosus |
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| Authors: | Ludwig Grü ter,Matthias Endres,Sö ren Gatermann |
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| Affiliation: | Institut für Medizinische Mikrobiologie und Immunologie, Universit?t Hamburg, FRG. |
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| Abstract: | The urease gene sequences of Staphylococcus epidermidis CNS23 were cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned sequences revealed four polypeptides (60, 17, 11 and 7.5 kDa) that were associated with enzyme activity. Southern hybridisation experiments showed high homologies with the urease genes of Staphylococcus saprophyticus. |
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| Keywords: | Urease Staphylococcus epidermidis Staphylococcus cantosus Molecular cloning |
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