Evaluation of dimethylsulfoxide (DMSO) injury to the myocardial cell by determination of high-energy phosphate reserve and enzyme release |
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| Authors: | M Bercot M de Mendonca K Schwartz J Leandri J P Cachera |
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| Affiliation: | 1. Institute of Construction Management, School of Civil Engineering and Mechanics, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, China;2. Civil and Structural Engineering Consultants Pvt (Ltd), Colombo 3, Sri Lanka;1. Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Leiria, Campus 2–Morro do Lena, Alto Vieiro, Apartado 4163, Leiria 2411-901, Portugal;2. Department of Marketing and Communication, Athens University of Economics and Business12 Derigny Str., Athens 10434, Greece;3. Leeds University Business School, University of Leeds, Maurice Keyworth Building, Leeds LS2 9JT, United Kingdom |
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| Abstract: | Determinations of high-energy phosphates (PC and ATP) were carried out in rabbit hearts (perfused at 23 °C with a 2.1 hyperkalemic solution of DMSO for different periods of time), some of which underwent an additional washingout procedure and were reperfused.This procedure always caused modifications in the ATP and PC concentrations compatible with a good viability of the heart. The time of contact rather than concentration seems to be responsible for deterioration as long as DMSO concentration does not exceed 2.1 m/liter.Assays of enzymes (CPK, LDH) performed throughout the same procedure showed that the figures obtained were compatible with a brief but temporary modification of membrane permeability such as those induced by a hyperkalemic perfusion, but that the part played by DMSO in these modifications appears to be only slight in the majority of cases. |
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