cDNA approaches to isolation of the mouse mutant weaver gene |
| |
| Authors: | M. E. Hodes S. R. Dlouhy J. J. Wei Y. Wang L. Sangameswaran V. Lazar L. C. Triarhou B. Ghetti |
| |
| Affiliation: | (1) Department of Medical and Molecular Genetics, Indiana University School of Medicine, 46202-5251 Indianapolis, Indiana;(2) Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 46202-5251 Indianapolis, Indiana;(3) Program in Medical Neurobiology, Indiana University School of Medicine, 46202-5251 Indianapolis, Indiana |
| |
| Abstract: | The mouse autosomal recessive mutant gene weaver (wv) results in abnormalities in cerebellum, substantia nigra and testis. Although a subtracted cDNA library prepared by removing P31 (wv/wv) sequences from a P1 (wv/+) library should contain mainly nonrepetitive neonatal sequences, unfortunately, repetitive sequences still appear during screening. Two clones, one repetitive, the other not, are used to illustrate the problems encountered in attempting to isolate the weaver gene from a subtracted cDNA library.Special issue dedicated to Dr. Sidney Ochs. |
| |
| Keywords: | Weaver mutant mice granule cells sequence, in situ hybridization |
| 本文献已被 SpringerLink 等数据库收录！ |
|
