Isolation of anthglutin, an inhibitor of gamma-glutamyl transpeptidase from Penicillum oxalicum. |
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Authors: | S Minato |
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Institution: | Fermentation Research Laboratories, Sankyo Co., Ltd., Tokyo 140, Japan |
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Abstract: | Anthglutin, a new inhibitor of γ-glutamyl transpeptidase, has been isolated from the cultured medium of Penicillium oxalicum and its structure established as l-γ-l-glutamyl-2-(2-carboxyphenyl)hydrazine. The isolation of anthglutin was achieved by ion-exchange chromatography. Anthglutin inhibited γ-glutamyl transpeptidase specifically and the kinetic analysis of the inhibition showed that anthglutin inhibited the enzyme competitively with regard to the glutamyl donor, γ-glutamyl-p-nitroanilide, and noncompetitively with regard to the glutamyl acceptor, glycylglycine. K1 values were 5.7 μm for the hog kidney enzyme, 18.3 μm for the human kidney enzyme, 13.6 μm for the human liver soluble enzyme, and 10.2 μm for the bound enzyme. After oral administration of 14C]methionine and anthglutin to rats, no effect of anthglutin was observed on the absorption of methionine in the intestine. |
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