In vitro organogenesis and somatic embryogenesis from leaf explants of Leucosceptrum canum sm. |
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Authors: | Amita Pal Anjana Banerjee Krishna Dhar |
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Institution: | (1) Tissue Culture Section, Bose Institute Kankurgachi, 700 054 Calcutta, India |
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Abstract: | Plantlets were obtained from leaf explants of a Labiatae tree — Leucosceptrum canum Sm. using plant tissue culture techniques. Two types of calli proliferated from the leaf explants when grown on different media, one of which was amenable to somatic embryogenesis. Differentiation of the embryoids started from the fourth passage of culture and continued up to the seventh passage. The number of embryoids decreased with the age of the callus. The capacity of such embryoids to form entire plantlets was studied using different nutrient mileux. Embryoids formed plantlets on Murashige and Skoog's (MS) medium fortified with benzylaminopurine plus indolebutyric acid. Organogenesis was observed in shoot-buds derived from explants of in vitro regenerated plantlets on MS basal medium supplemented with benzylaminopurine. Culture regenerated plantlets were transferred to MS medium without sucrose and growth hormones; finally transferred to pots containing sterile vermiculite where they are growing.Abbreviations MS
Murashige and Skoog's medium
- 2,4-D
2,4-dichlorophenoxy acetic acid
- NAA
naphthaleneacetic acid
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- Kn
kinetin
- BAP
benzylaminopurine
- CW
coconut water |
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Keywords: | |
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