首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Fast and precise protein tracking using repeated reversible photoactivation
Authors:Chudakov Dmitriy M  Chepurnykh Tatyana V  Belousov Vsevolod V  Lukyanov Sergey  Lukyanov Konstantin A
Institution:Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. chudakovdm@mail.ru
Abstract:Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
Keywords:asCP  asFP595  asulCP  Bid  photoactivatable protein  protein movement tracking  reversible photoactivation
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号