人体子宫内膜中性鞘糖脂和神经节苷脂的含量和组成在月经周期中和妊娠时的变化

从月经周期各期、早妊(6—8周)及足月妊娠子宫内膜中提取、纯化了神经节苷脂(Gls)和中性鞘糖脂(N-GSL),分别测定了其含量。对两类鞘糖脂的组成进行了HPTLC图谱分析。初步观察了不同时期子宫内膜中CMP—NeuAc:LacCer唾液酰转移酶(ST_1)和CMP-NeuAc:CM_3唾液酰转移酶(ST_2)的活性。结果表明:分泌期的GLs总含量低于增生期(P<0.02);分泌期GD_3含量较生长期增多(P<0.01);妊娠后,GM_3含量增加,而GD_3减少;相应地,ST_1活性增高,ST_2活性降低。分泌期CMH含量为增生期的4.7倍。结果提示子宫内膜鞘糖脂含量和组成的变化可能与子宫的功能有关,而且受女性激素水平的影响。

Changes of Contents and Compositions of Neutral Glycosphingo-Iipid and Ganglioside in Human Endometrium at Various Stages of Menstrual Cycle and Pregnancy

Ganglioside(Gls) and neutral glycosphingolipid(N-GSL) were extracted and purified from human endometrium at various stages of menstrual cycle and pregnancy. The sialic acid contents of Gls and sphingosine contents of N-GSL were quan titated respectively. The compositions of Gls and N-GSL were analyzed by HPTLC. Activities of CMP-NeuAc:LacCer sialytransferase(ST1) and CMP-NeuAc:GM3 sialy Itransferase (ST2) in endometrium at different stages were assayed. The content of the sialic acid in secretory endometrium was much lower than in the proliferous(P< 0.02) .The content of GD3 in secretory-endometrium was significantly higher than that in the proliferous (P<0.01) .After pregnancy, the content of GM3 increased, but tnat of GD3 decreased; while the activity of ST1 increased and tne activity of ST2 decreased. The content of CMH in secretory endometrius was 4.7 times higher than that in the proliferous .The results suggest that the changes of contents and compositions of glycosphingolipids might be related to the function of endometrium, and that female hormones might influence the metabolism of glycosphingolipid.