Staufen基因在结直肠癌组织中的表达

为研究甲基化差异相关基因Staufen在结直肠癌不同组织中的表达情况，采用RT-PCR和免疫组织化学等方法在结直肠癌病人中检测Staufen基因在腺癌、癌旁粘膜和相应远端切缘正常组织中的表达。研究发现，在mRNA水平上，远端切缘正常组织中Staufen基因的表达水平显著高于癌旁粘膜和腺癌（P<0.05）；从正常组织、癌旁粘膜到腺癌，Staufen基因的表达有降低的趋势；未发现性别、年龄、肿瘤部位（结肠/直肠）、分化程度、淋巴结转移等临床病理因素与Staufen基因的表达有关（P均>0.05）。正常组织与腺癌组织的Staufen蛋白的表达水平显著高于癌旁粘膜（P<0.05），而正常组织与腺癌组织之间未发现有统计学差别（P>0.05）。结果表明，Staufen基因在癌旁粘膜和肿瘤中有低表达的趋势，该基因可能在结直肠癌的发生、发展中起作用。

The Expression of Staufen Gene in Colorectal Cancer

To study the expression of differential methylation - associated Staufen gene in colorectal cancer(CRC), we detected the expression of Staufen gene in adenocarcinoma, mucosa adjacent to colorectal cancer(MACC)and corresponding distant normal tissue using RT-PCR and immunohistochemistry. We found that the expression of Staufen gene in MACC and adenocarcinoma of CRC was significantly lower than that in corresponding distal normal tissue at mRNA level(P<0.05).No significant associations were found between age, sex, location of cancer (colon/ rectal), degree of differentiation and metastasis of lymph node with expression of Staufen gene. Lower expression of Staufen protein in MACC was verified by comparing with matched normal tissue and adenocarcinoma(P<0.05). This study indicated low expression of Staufen gene in colorectal cancer and the Staufen gene might be involved in the development of colorectal cancer.