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Separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence |
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| Authors: | A S Paau J R Cowles J Oro A Bartel E Hungerford |
| Institution: | (1) Department of Biology, University of Houston, 77004 Houston, Texas, USA;(2) Department of Biophysical Sciences, University of Houston, 77004 Houston, Texas, USA;(3) Department of Physics, University of Houston, 77004 Houston, Texas, USA |
| Abstract: | The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid.Abbreviation FMF Flow-microfluorometer |
| Keywords: | Flow-microfluorometry Separation Microbial cells Chlorophyll fluorescence Ethidium bromide Nucleic acid |
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