In vivo incorporation of cytidine-monophosphate-ciliatine into rat liver lipids. |
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Authors: | M Tamari A Cassaigne A M Lacoste E Neuzil |
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Affiliation: | Laboratoire de Biochimie, U.E.R. III, Université de Bordeaux II, 146, rue Léo Saignat, 33076 Bordeaux, France |
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Abstract: | 1. In vivo this investigation was carried out in order to compare the incorporation into rat lipids of free [1,2-minus 14C]-ciliatine and CMP-[1,2-minus 14C]-ciliatine which is the precursor in phosphonolipid biosynthesis. 2. The incorporation of the radioactivity from CMP-[1,2-minus 14C]-ciliatine took place more rapidly than that from free [1,2-minus 14C]-ciliatine in both liver and kidney. The amount of radioactivity from the CMP-[1,2-minus 14C]-ciliatine incorporated into total liver lipids was about 5 times higher than that incorporated into total liver lipids of rat two hrs after injecting free-[1,2-minus 14C]-ciliatine. 3. The amount of [1,2-minus 14C]-ciliatine incorporated into total liver lipids was 15 and 21 times higher than that incorporated into total kidney lipids of rat two and four hrs after injecting free [1,2-minus 14C]-ciliatine. 4. If the main pathway for the phosphonolipid biosynthesis is via CMP-ciliatine, the rate of phosphonolipid formation from CMP-ciliatine must therefore be higher than that from free-ciliatine. The results obtained here indicate therefore that the main pathway for phosphonolipid biosynthesis is a pathway involving CMP-ciliatine. 5. An unknow compound was detected in the water soluble fraction of the acid hydrolyzate of liver phosphonolipids. This material migrated with the N-trimethyl-derivative of ciliatine on the thin-layer chromatogram. The result shows that there is therefore a possibility of methylation of exogenous ciliatine to the phosphonate analogue of choline in the mammalian body. |
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Keywords: | Ciliatine 2-aminoethylphosphonic acid (2-AEP) CMP Cytidine-5′-monophosphate CMP-ciliatine Cytidine monophosphate-2-aminoethylphosphonic acid CDP-ethanolamine Cytidine diphosphate-ethanolamine CDP-choline Cytidine diphosphate-choline DCC N,N'-dicyclohexylcarbodiimide |
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