离子注入对萝卜过氧化物酶、淀粉酶和蛋白酶同工酶的影响

研究了氮离子和氩离子注入萝卜种子对萝卜幼苗蛋白含量、过氧化物酶活性及过氧化物酶、淀粉酶和蛋白酶同工酶的影响。结果表明 :离子注入后 ,减低萝卜过氧化物酶活性和蛋白含量。萝卜不同生长时期同工酶变化不一样。在子叶时期 ,过氧化物酶同工酶谱带无明显变化 ,淀粉酶同工酶有酶带的消失 ;而在真叶时期 ,过氧化物酶在负极区减少一条酶带 ,Rf为 0 .2 2 ,正极区增加一条酶带 ,Rf为 0 .6 ,且随剂量增加 ,酶带着色增强 ;淀粉酶同工酶在注入剂量为 5× 10 5N+ / cm2 )时 ,有同工酶带增加 ,Rf为 0 .6 1。低剂量时蛋白酶活性增强 ,谱带增多 ,大剂量则减弱。因此 ,N+和 Ar+注入后 ,可影响萝卜过氧化物酶和淀粉酶的表达及蛋白质的合成或降解。

Effects of ion implantation on peroxidase,amylase and protease isoenzyme in radish

WT5”BZ] The effects of ion implantation on contents of soluble protein, activities of peroxidase and peroxidase, amylase and protease isoenzyme pattern in radish were studied. The results show that: after ion implantation, contents of soluble protein and activities of peroxidase in the experimental. group are apparently lower than in the control group, Besides, there are differences of isoenzyme pattern during different growth period. During cotyledon period, peroxidase isoenzyme pattern had not obviously changed, but a band of amylase isoenzyme pattern disappeared. During euphylis period, a band (Rf 0 22)of peroxidase isoenzyme disappeared in the positive electrode area. and the intencity of peroxidase isoenzyme band is enhanced as the ion implantation dose increased. A new band (Rf: 0 61) of amylase isoenzyme has been occupied at the ion implantation dose of 5×10 5 N +/cm 2. Low dose of ion implantation could enhance activity and increase bands of protease isoenzyme. These suggest that: N + and Ar + ion implantation can affect the expression of peroxidase or amylase and the synthesis and degradation of proteins.