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填充床反应器中酶法连续合成甘油二酯的研究
引用本文:孟祥河, 孙培龙, 杨开, 何荣军, 毛忠贵,. 填充床反应器中酶法连续合成甘油二酯的研究[J]. 生物工程学报, 2005, 21(3): 425-429
作者姓名:孟祥河   孙培龙   杨开   何荣军   毛忠贵  
作者单位:1. 浙江工业大学生物与环境学院,杭州,210032
2. 江南大学生物工程学院,无锡,214036
摘    要:近年来,1,3-甘油二酯(DAG)由于其广泛用途及健康作用日益受到人们的重视。报道了一种无溶剂条件下填充床反应器中连续酶促合成1,3-DAG的方法。研究了填充柱的长径比、进料体积流速、温度、底物摩尔比对酯化率和1,3-DAG产量的影响。结果表明固定化酶填充柱长径比7.8,亚油酸、甘油摩尔比1∶2 ,进料速度1.2mL/min ,65℃条件下酯化反应可实现脂肪酸酯化率、1,3-DAG纯度及生产效率的统一。填充床反应器中固定化酶连续催化酯化反应的一个主要问题即体系水分清除困难。实验研究了采用过量甘油吸附脱水的可行性,亚油酸、甘油摩尔比为1∶2时,可明显改善固定化酶的稳定性,增加LipozymeRMIM的使用寿命。连续运行10d ,残余酶活仍保持在80 %以上,而对照组则仅为52%。

关 键 词:1   3-甘油二酯   合成   Lipozyme RM IM   填充柱反应器   脱水  
文章编号:1000-3061(2005)03-0425-05
修稿时间:2004-11-16

Synthesis of Diacylglycerol Using Immoblized 1, 3-Regiospecific Lipase in Continuously Operated Fixed Bed Reactors
MENG Xiang-He,SUN Pei-long,YANG Kai,HE Rong-jun,MAO Zhong-gui. Synthesis of Diacylglycerol Using Immoblized 1, 3-Regiospecific Lipase in Continuously Operated Fixed Bed Reactors[J]. Chinese journal of biotechnology, 2005, 21(3): 425-429
Authors:MENG Xiang-He  SUN Pei-long  YANG Kai  HE Rong-jun  MAO Zhong-gui
Affiliation:College of Biological & Environmental Engineering, Zhejiang University of Technology, Hangzhou 210032, China.
Abstract:Diacylglycerol, DAG, because of its multifunctional and nutritional properties, attracted considerable attention recently. Enzymatic synthesis of diacylglycerols from linoleic acid was investigated in a solvent-free reaction in a continuously operated fixed bed reactors containing Lipozyme RM IM. By appropriate manipulation of the fluid-residence time, the relative proportions of the various acylglycerols in the effluent stream can be controlled. In addition, the presence of excess glycerol is effective for the removal of water produced during the esterification reactions. Under the conditions of molar ratio of linoleic acid to glycerol of 0.5, the immoblized enzyme maintained high stability and allowed the reaction to continue for 10 days without significant deterioration in enzyme activity. It was determined that the conversion of fatty acid, content of 1,3-DAG and volume efficiency of reactor reached optima under the conditions: a packaged-bed reactor(with a ratio of packed length to inner diameter of 7.8), reacting temperature at 65 degrees C, molar ratio of linoleic acid to glycerol of 0.5, and feeding flow rate of 1.2 mL/min.
Keywords:1  d-d-Diacylglycerol   synthesis   lipozyme RM IM   fixed bed reactor   water removal
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