Quantitation of estrogen receptors and relaxin binding in human anterior cruciate ligament fibroblasts |
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Authors: | Deborah A Faryniarz Madhu Bhargava Claudette Lajam Erik T Attia Jo A Hannafin |
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Institution: | (1) Department of Orthopedic Surgery and Sports Medicine, The Permanente Medical Group, Inc., Santa Clara, California;(2) Sports Medicine and Shoulder Service, Hospital for Special Surgery, New York;(3) Department of Orthopaedic Surgery, Mayo Clinic, Rochester, Minnesota |
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Abstract: | Summary The significantly higher incidence of anterior cruciate ligament (ACL) injuries in collegiate women compared with men may
result from relative ligament laxity. Differences in estrogen and relaxin activity, similar to that seen in pregnancy, may
account for this. We quantified estrogen receptors by flow cytometry and relaxin receptors by radioligand binding assay in
human ACL cells and compared the presence of these receptors in males and females. ACL stumps were harvested from seven males
and eight females with acute ACL injuries. The tissue was placed in M199 cell culture medium. Outgrowth cultures were obtained,
and passage 2 cells were used for all studies. Estrogen receptor determination was performed using flow cytometry. Relaxin
binding was performed in ACL cells derived from five female and male patients using I125-labeled relaxin. Estrogen receptors were identified by flow cytometry in 4 to 10% of ACL cells. Mean fluorescence of cells
expressing estrogen receptors was approximately twice that of controls, with no significant differences between males and
females. Relaxin studies showed low-level binding of I125-relaxin-labeled ACL cells. Relaxin binding was present in four out of five female ACL cells versus one out of five male ACL
cells. |
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Keywords: | flow cytometry gender estrogen receptor relaxin anterior cruciate ligament binding |
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