Isolation and Characterization of Microsatellite Loci for Striped Bass (Morone saxatilis) |
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Authors: | Kaiping Han Li Li Gilles M Leclerc Alison M Hays Bert Ely |
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Institution: | (1) FISHTEC Genetics Laboratory, Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, U.S.A., US |
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Abstract: | Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass.
Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea
bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations
of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to
0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic
markers developed for striped bass.
Received November 9, 1999; accepted February 11, 2000. |
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Keywords: | : Striped bass Morone saxatilis polymorphism microsatellites |
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