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法国冬青和光皮桦挥发物日节律及云斑天牛的触角电位反应
引用本文:杨桦,杨伟,杨茂发,杨春平,杨令国,徐唐鑫科. 法国冬青和光皮桦挥发物日节律及云斑天牛的触角电位反应[J]. 应用生态学报, 2011, 22(2)
作者姓名:杨桦  杨伟  杨茂发  杨春平  杨令国  徐唐鑫科
作者单位:1. 四川农业大学森林保护重点实验室,四川雅安625014;贵州大学昆虫研究所贵州山地农业病虫害重点实验室,贵阳550025
2. 四川农业大学森林保护重点实验室,四川雅安,625014
3. 贵州大学昆虫研究所贵州山地农业病虫害重点实验室,贵阳,550025
4. 罗江县林业局,四川罗江,618500
基金项目:四川省教育厅重点实验室专项(2006ZD011); 四川农业大学“211工程”双支计划项目(00370101),四川农业大学长江上游生态林业工程建设项目资助
摘    要:采用Tenax-TA吸附剂吸附法结合气相色谱-质谱联用技术(GC-MS)分析了法国冬青和光皮桦的健康株和云斑天牛危害株挥发性物质的日节律变化,并利用触角电位技术比较分析了云斑天牛未交配雌、雄虫对法国冬青和光皮桦挥发物的触角电位(EAG)反应.结果表明:法国冬青被取食后柠檬烯、壬醛、十六烷、丙烯酸丁酯和3-甲基丁酸的含量减少,光皮桦被取食后新己烷和十六烷的含量减少;而法国冬青和光皮桦被取食后新合成了异十二烯、辛醇、吲哚、癸醛、己醛和丙烯菊酯等物质.云斑天牛未交配雌、雄成虫对光皮桦被害株挥发物的EAG反应值最大,分别为1.23和1.38 mV;对法国冬青健康株挥发物的EAG反应值最小,分别为0.95和1.01 mV.不同时段,成虫对12:00—14:00挥发成分的EAG反应值最低,与成虫野外取食行为多发生在清晨和傍晚相一致.

关 键 词:法国冬青  光皮桦  植物挥发物  日节律  生理状态  触角电位反应  

Diurnal rhythm of Viburnum awabuki and Betula luminifera volatiles and electroantennogram response of Batocera horsfiddi.
YANG Hua,YANG Wei,YANG Mao-fa,YANG Chun-ping,YANG Ling-guo,XUTANG Xin-ke. Diurnal rhythm of Viburnum awabuki and Betula luminifera volatiles and electroantennogram response of Batocera horsfiddi.[J]. The journal of applied ecology, 2011, 22(2)
Authors:YANG Hua  YANG Wei  YANG Mao-fa  YANG Chun-ping  YANG Ling-guo  XUTANG Xin-ke
Affiliation:YANG Hua1,2,YANG Wei1,YANG Mao-fa2,YANG Chun-ping1,YANG Ling-guo3,XUTANG Xin-ke3(1Key Laboratory of Forest Conservation,Sichuan Agricultural University,Ya'an 625014,Sichuan,China,2Key Laboratory for Plant Pest Management of Mountain Region,Institute of Entomology,Guizhou University,Guiyang 550025,3Luojiang Forestry Bureau,Luojiang 618500,China)
Abstract:By the method of Tenax-TA absorbent adsorption combined with GC-MS,this paper analyzed the changes of the diurnal rhythm of the volatiles in the healthy branches and Batocera horsfieldi-damaged branches of Viburnum awabuki and Betula luminifera,and electroantennogram technique was used to perform a comparative analysis on the electroantennogram(EAG) responses of unmated male and female B.horsfieldi to the volatiles.After the feeding by B.horsfieldi,there was a decrease in the contents of limonene,nonanal,he...
Keywords:Viburnum awabuki  Betula luminifera  plant volatiles  diurnal rhythm  physiological status  electroantennogram response.  
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